Neuroanatomical tracing methods remain fundamental for elucidating the complexity of brain circuits. During the past decades, the technical arsenal at our disposal has been greatly enriched, with a steady supply of fresh arrivals. This paper provides a landscape view of classical and modern tools for tract-tracing purposes. Focus is placed on methods that have gone viral, i.e., became most widespread used and fully reliable.
Jose L. Lanciego; Floris G. Wouterlood - Neurosciences Department, Center for Applied Medical Research (CIMA), University of Navarra, Pamplona, Spain / Department of Anatomy and Neurosciences, Amsterdam University Medical Centers, Amsterdam, The Netherlands
How Amira-Avizo Software is used
Slides […] were studied in a confocal laser scanning microscope. Structures were 3D rendered using Amira™ software (Thermo Fisher Scientific).