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Neuroanatomical tracing methods remain fundamental for elucidating the complexity of brain circuits. During the past decades, the technical arsenal at our disposal has been greatly enriched, with a steady supply of fresh arrivals. This paper provides a landscape view of classical and modern tools for tract-tracing purposes. Focus is placed on methods that have gone viral, i.e., became most widespread used and fully reliable.
Slides […] were studied in a confocal laser scanning microscope. Structures were 3D rendered using Amira™ software (Thermo Fisher Scientific).
For Research Use Only. Not for use in diagnostic procedures.