Ptychographic X-ray CT characterization of the osteocyte lacuno-canalicular network in a male rat's glucocorticoid induced osteoporosis model

Antonia Ciani, Hechmi Toumi, Stéphane Pallu, Esther H.R.Tsai, Ana Diaz, Manuel Guizar-Sicairos, Mirko Holler, Eric Lespessailles, Cameron M.Kewish | Synchrotron Soleil, France

Ptychographic X-ray computed tomography (PXCT) is a quantitative imaging modality that non-destructively maps the 3D electron density inside an object with tens of nanometers spatial resolution. This method provides unique access to the morphology and structure of the osteocyte lacuno-canalicular network (LCN) and nanoscale density of the tissue in the vicinity of an osteocyte lacuna.  Our findings indicate that PXCT can non-destructively provide detailed, nanoscale information on the 3D organization of the LCN in correlative studies of pathologies, such as osteoporosis, leading to improved diagnosis and therapy.

How Amira-Avizo Software is used

A preliminary gray-level thresholding together with fill and closing operations, masks the air background around the sample and selects only the bone mineral matrix and LCN for segmentation using Avizo 3D software (Avizo 3D Software|FEI Software, n.d.).