Three-dimensional imaging of the intracellular assembly of a functional viral RNA replicase complex

Isabel Fernández de Castro, José J. Fernández, Daniel Barajas, Peter D. Nagy, Cristina Risco

Positive-strand RNA viruses, which can be devastating pathogens in humans, animals and plants, replicate their genomes on intracellular membranes. Here, we describe the three-dimensional ultrastructural organization of a tombusvirus replicase in yeast, a valuable model for exploring virus–host interactions…

How Amira-Avizo Software is used

Noise was reduced with three rounds of median filter, segmentation and 3D visualization using Amira software.