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Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy

Qinyi Fu, Benjamin L. Martin, David Q. Matus, Liang Gao

Despite the progress made in selective plane illumination microscopy, high-resolution 3D live imaging of multicellular specimens remains challenging. Tiling light-sheet selective plane illumination microscopy (TLS-SPIM) with real-time light-sheet optimization was developed to respond to the challenge…

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