Multiscale reconstruction of various vessels in the intact murine liver lobe
Qi Zhang, Anan Li, Siqi Chen, Jing Yuan, Tao Jiang, Xiangning Li, Qingming Luo,Zhao Feng & Hui Gon - Britton Chance Center for Biomedical Photonics/Wuhan National Laboratory for Optoelectronics/MoE Key Laboratory for Biomedical Photonics/Huazhong University of Science and Technology, Wuhan, China HUST-Suzhou Institute for Brainsmatics, Suzhou, China Hainan University, Haikou, China
The liver contains a variety of vessels and participates in miscellaneous physiological functions. While past studies generally focused on certain hepatic vessels, we simultaneously obtained all the vessels and cytoarchitectural information of the intact mouse liver lobe at single-cell resolution. […] providing a technology roadmap for studying the fine hepatic vascular structures and their spatial relationship, which will help research into liver diseases and evaluation of medical efficacies in the future.
![Multiscale reconstruction of various vessels in the intact murine liver lobe]()
In this article, we used Tek-Cre*Ai47 mice to acquire the vessel
structures and cytoarchitecture information of intact liver lobe
with single-cell resolution, based on propidium iodide staining
and the HD-fMOST system. According to the morphology discrepancies among different vessels, we segmented large-caliber
vessels, namely portal vein, hepatic vein, hepatic artery,
intrahepatic bile ducts, and lymphatic vessels, and reconstructed
hepatic sinusoids and peribiliary plexus in local areas.
How Amira-Avizo Software is used
We used the Amira software to manually segment the structure of the peribiliary plexus (Fig. 5b, c) […] Amira software (v.6.1.1, FEI) was used to distinguish hepatic vein and portal vein, and to correct errors such as missing or misidentified branches based on the cytoarchitecture information. Manual tracing of hepatic artery, bile ducts, and lymphatic vessels was also executed with the Amira […] The skeletonization of sinusoids was performed with the Amira software. The detection of the diameter of hepatic sinusoids was executed with the rayburst algorithm24 […] We used Amira to manually reconstruct the local peribiliary vascular plexus on the merged images of two channels […] The filament editor module, segmentation editor, and autoskeleton tool of Amira software were respectively used in vessel tracing, manual segmentation of peribiliary plexus, and sinusoidal skeletonization.
