Welcome to the Amira-Avizo Software Use Case Gallery
Below you will find a collection of use cases of our 3D data visualization and analysis software. These use cases include scientific publications, articles, papers, posters, presentations or even videos that show how is used to address various scientific and industrial research topics.
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Multicellular tumour spheroids are used as a culture model to reproduce the 3D architecture, proliferation gradient and cell interactions of a tumour micro-domain. However, their 3D characterization at the cell scale remains challenging due to size and cell density issues. In this study, we developed a methodology based on 3D light sheet fluorescence microscopy (LSFM) image analysis and convex hull calculation that allows characterizing the 3D shape and orientation of cell nuclei relative to ... Read more
Annaïck Desmaison, Ludivine Guillaume, Sarah Triclin, Pierre Weiss, Bernard Ducommun & Valérie Lobjois
The size and growth patterns of the components of the human embryonic heart have remained largely undefined.
To provide these data, three-dimensional heart models were generated from immunohistochemically stained sections of ten human embryonic hearts ranging from Carnegie stage 10 to 23. Fifty-eight key structures were annotated and volumetrically assessed. Sizes of the septal foramina and atrioventricular canal opening were also measured. The heart grows exponentially throughout embr... Read more
Jaeike W. Faber, Jaco Hagoort, Antoon F. M. Moorman, Vincent M. Christoffels, Bjarke Jensen
Cell-to-cell contact is essential for communication and development of multicellular organisms. A prerequisite is the passage through membranes. That way, molecular exchange and information flow is regulated via hormones, membrane proteins and pores.
In plants, the rigid cell walls prevent large membrane contact areas between protoplasts. Only plasmodesmata, minute channels between adjacent cells, form direct connections. Often, molecular data of the proteins involved are manifold but t... Read more
Dominik Harant and Ingeborg Lang
The mitotic spindle ensures the faithful segregation of chromosomes. Here we combine the first large-scale serial electron tomography of whole mitotic spindles in early C. elegans embryos with live-cell imaging to reconstruct all microtubules in 3D and identify their plus- and minus-ends. We classify them as kinetochore (KMTs), spindle (SMTs) or astral microtubules (AMTs) according to their positions, and quantify distinct properties of each class. While our light microscopy and muta... Read more
Stefanie Redemann, Johannes Baumgart, Norbert Lindow, Michael Shelley, Ehssan Nazockdast, Andrea Kratz, Steffen Prohaska, Jan Brugués, Sebastian Fürthauer & Thomas Müller-Reichert
Until March 2021, around 120 million coronavirus disease (COVID-19) infected cases and over 2.6 million deaths have been reported worldwide. […] Recent investigations have implied that face masks help to reduce the disease transmission and therefore slow down the growth of the epidemic curve. However, there are still ongoing debates on the efficacy of wearing masks […] since there is a general lack of information relating to the material structure of commonly used face masks.Read more
Wenjia Du, Francesco Iacoviello, Tacson Fernandez, Rui Loureiro, Daniel J. L. Brett & Paul R. Shearing
Progressive transformation of the otic placode into the functional inner ear during gestational development in humans leads to the acquisition of hearing perception via the cochlea and balance and spatial orientation via the vestibular organ.
Using a correlative approach involving micro-computerized tomography (micro-CT), transmission electron microscopy and histological techniques we were able to examine both the morphological and cellular changes associated with human inner ear devel... Read more
Lejo Johnson Chacko, David Wertjanz, Consolato Sergi, Jozsef Dudas, Natalie Fischer, Theresa Eberharter, Romed Hoermann, Rudolf Glueckert, Helga Fritsch, Helge Rask-Andersen, Anneliese Schrott-Fischer & Stephan Handschuh
Inconel 718 (IN718) is the most popular precipitation-strengthened nickel-based superalloy introduced by the Huntington Alloys Division of INCO in 1959 (Ref Read more
Oskar Dziuba, Grzegorz Cempura, Agnieszka Wusatowska-Sarnek & Adam Kruk
Alternative battery technologies are required to meet growing energy demands and address the limitations of present technologies. As such, it is necessary to look beyond lithium-ion batteries. Zinc batteries enable high power density while being sourced from ubiquitous and cost-effective materials. This paper presents, for the first time known to the authors, multi-length scale tomography studies of failure mechanisms in zinc batteries with and without commercial microporous separators. In bo... Read more
Vladimir Yufit, Farid Tariq David S. Eastwood Moshiel Biton Billy Wu Peter D. Lee Nigel P. Brandon
In aqueous solutions, electro/chemically deposited metals usually grow outward into electrolyte. Here we report that the reduced Al grows inward into the sample, surprisingly, while Mg (in pure Mg and Al2Mg3 alloy) is galvanically replaced with Al in an ionic liquid. The galvanic replacement reaction (GRR) of Al2Mg3 involves a dealloying process that generates a nanoporous Al skeleton, and simultaneously the inward-growth plating of Al that thicke... Read more
Wei Yang, Xian-Gui Zheng, Shao-Gang Wang, Hai-Jun Jin
Metal-organic frameworks (MOFs) and metal nanoparticles are two classes of materials that have received considerable recent attention, each for controlling chemical reactivities, albeit in very different ways. Here, we report the growth of MOF shell layers surrounding aluminum nanocrystals (Al NCs), an Earth-abundant metal with energetic, plasmonic, and photocatalytic properties. The MOF shell growth proceeds by means of dissolution-and-growth chemistry that uses the intrinsic surface oxide o... Read more
Hossein Robatjazi, Daniel Weinberg, Dayne F. Swearer, Christian Jacobson, Ming Zhang, Shu Tian, Linan Zhou, Peter Nordlander, Naomi J. Halas
Catalyst aging effects were analyzed using X-ray absorption micro-computed tomography in combination with conventional characterization methods on various length scales ranging from nm to μm to gain insight into deactivation mechanisms.
For this purpose, a 4 wt% Pt/Al2O3 model exhaust gas catalyst was coated on a cordierite honeycomb and subjected to sequential thermal aging in static air at 950 °C for 4, 8, 12 and 24 hours. The ag... Read more
Georg Hofmann, Amélie Rochet, Elen Ogel, Maria Casapu, Stephan Ritter, Malte Ogurreck and Jan-Dierk Grunwaldt
Intracellular pathogens include all viruses, many bacteria and parasites capable of invading and surviving within host cells. Key to survival is the subversion of host cell pathways by the pathogen for the purpose of propagation and evading the immune system. The intracellular bacterium Shigella flexneri, the causative agent of bacillary dysentery, invades host cells in a vacuole that is subsequently ruptured to allow growth of the pathogen within the host cytoplasm…Read more
Allon Weiner , Nora Mellouk , Noelia Lopez-Montero , Yuen-Yan Chang, Célia Souque, Christine Schmitt, Jost Enninga
Evidence for macroscopic life in the Paleoproterozoic Era comes from 1.8 billion-year-old (Ga) compression fossils [Han TM, Runnegar B (1992) Science 257:232–235; Knoll et al. (2006) Philos Trans R Soc Lond B 361:1023–1038], Stirling biota [Bengtson S et al. (2007) Paleobiology 33:351–381], and large colonial organisms exhibiting signs of coordinated growth from the 2.1-Ga Francevillian series, Gabon. Here we report on pyritized string-shaped structures from... Read more
Abderrazak El Albani, M. Gabriela Mangano, Luis A. Buatois, Stefan Bengtson, Armelle Riboulleau, Andrey Bekker, Kurt Konhauser, Timothy Lyons, Claire Rollion-Bard, Olabode Bankole, Stellina Gwenaelle Lekele Baghekema, Alain Meunier, Alain Trentesaux, Arnaud Mazurier, Jeremie Aubineau, Claude Laforest, Claude Fontaine, Philippe Recourt, Ernest Chi Fru, Roberto Macchiarelli, Jean Yves Reynaud, François Gauthier-Lafaye, and Donald E. Canfield
The tissue inhibitor of metalloproteinases-1 (TIMP-1) exerts inhibitory activity against matrix metalloproteinases and cytokine-like effects. We previously showed that TIMP-1 reduces neurite outgrowth in mouse cortical neurons and that this cytokine-like effect depends on TIMP-1 endocytosis mediated by the low-density lipoprotein receptor-related protein-1 (LRP-1). To gain insight into the interaction between TIMP-1 and LRP-1, we considered conformational changes that occur when a ligand bind... Read more
Laurie Verzeaux, Nicolas Belloy, Jessica Thevenard-Devy, Jérôme Devy, Géraldine Ferracci, Laurent Martiny, Stéphane Dedieu, Manuel Dauchez, Hervé Emonard, Nicolas Etique & Emmanuelle Devarenne-Charpentier
Endothelial cells and pericytes are integral cellular components of the vasculature with distinct interactive functionalities. To study dynamic interactions between these two cells we created two transgenic animal lines. A truncated eNOS (endothelial nitric oxide synthase) construct was used as a GFP tag for endothelial cell evaluation and an inducible Cre-lox recombination, under control of the Pdgfrb (platelet derived growth factor receptor beta) promoter, was created for pericyte assessmen... Read more
Ann L. B. Seynhaeve, Douwe Oostinga, Rien van Haperen, Hanna M. Eilken, Susanne Adams, Ralf H. Adams & Timo L. M. ten Hagen
Laser-matter interactions in laser additive manufacturing (LAM) occur on short time scales (10-6 – 10-3 s) and have traditionally proven difficult to characterise. We investigate these interactions during LAM of stainless steel (SS316 L) and 13-93 bioactive glass powders using a custom built LAM process replicator (LAMPR) with in situ and operando synchrotron X-ray radiography. This reveals a range of melt track solidification phenomena as... Read more
Chu Lun Alex Leung, Sebastian Marussi, Michael Towrie, Jesus del Val Garcia, Robert C. Atwood, Andrew J. Bodey, Julian R. Jones, Philip J. Withers, Peter D.Lee
The near-threshold behavior of long cracks is evaluated using pre-cracked flat dogbone specimens of a commercial aluminum alloy in two heat treatment states. Once the threshold was known, the crack initially introduced by cyclic compression was propagated under load control with approximately
constant range of the stress intensity factor at values close to the threshold values. The 3D morphology of the crack and the spatial distribution of the primary precipitates obtained by µ-CT were... Read more
Marcel Wicke, Angelika Brueckner-Foit, Tina Kirsten, Martina Zimmermann, Fatih Buelbuel, Hans-Juergen Christ
Rapeseed is one of the most important and widely cultured oilseed crops for food and nonfood purposes worldwide. Neutral lipids are stored in lipid droplets (LDs) as fuel for germination and subsequent seedling growth. Most of the LD detection in seeds was still in 2D levels, and some of the details might have been lost in previous studies. In the present work, the configuration of LDs in seeds was obtained by confocal imaging combined with 3D reconstruction technology in Brassica napus<... Read more
Yongtai Yin, Liangxing Guo, Kang Chen, Zhenyi Guo, Hongbo Chao, Baoshan Wang, and Maoteng Licorresponding author
The microstructure of food affects our sensorial perception, its attractiveness, and the manufactured product’s shelf-life.
Microstructural evolution in soft matter directly influences not only the material’s mechanical and functional properties, but also our perception of that material’s taste. Using synchrotron X-ray tomography and cryo-SEM we investigated the time–temperature evolution of ice cream’s microstructure. This was enabled via three adv... Read more
Enyu Guo, Guang Zeng, Daniil Kazantsev, Peter Rockett, Julian Bent, Mark Kirkland, Gerard Van Dalen, David S. Eastwood, David StJohn and Peter D. Lee
How do you characterise the contents of a sealed nuclear waste package without breaking it open?
This question is important when the contained corrosion products are potentially reactive with air and radioactive. Synchrotron X-rays have been used to perform micro-scale in-situ observation and characterisation of uranium encapsulated in grout; a simulation for a typical intermediate level waste storage packet. X-ray tomography and X-ray powder diffraction generated both qualitative and ... Read more
C.A. Stitt, M. Hart, N.J. Harker, K.R. Hallam, J. MacFarlane, A. Banos, C. Paraskevoulakos, E. Butcher, C. Padovani, T.B. Scott
Ali Ertürk and his collaborators described a novel histo-chemical technique to clear spinal cord tissue of adult mice for fluorescence microscopy imaging of the intact spinal cord.
Previously, the high lipid content of the spinal cord tissue in adult mice allowed imaging of the spinal cord only through destructive histological methods, making the tracing of complete axons impossible. As applied in this study, the improved histo-chemistry enabled Ertürk and his colleagues to show that... Read more
Ali Ertürk, Christoph P Mauch, Farida Hellal, Friedrich Förstner, Tara Keck, Klaus Becker, Nina Jährling, Heinz Steffens, Melanie Richter, Mark Hübener, Edgar Kramer, Frank Kirchhoff, Hans Ulrich Dodt & Frank Bradke