Welcome to the Amira-Avizo Software Use Case Gallery

Below you will find a collection of use cases of our 3D data visualization and analysis software. These use cases include scientific publications, articles, papers, posters, presentations or even videos that show how Amira-Avizo Software is used to address various scientific and industrial research topics.

Use the Domain selector to filter by main application area, and use the Search box to enter keywords related to specific topics you are interested in.

Using micro-computed tomography to reveal the anatomy of adult Diaphorina citri Kuwayama (Insecta: Hemiptera, Liviidae) and how it pierces and feeds within a citrus leaf

Using micro-computed tomography to reveal the anatomy of adult Diaphorina citri Kuwayama (Insecta: Hemiptera, Liviidae) and how it pierces and feeds within a citrus leaf

The Asian citrus psyllid (ACP), Diaphorina citri, is a harmful pest of citrus trees that transmits Candidatus Liberibacter spp. which causes Huanglongbing (HLB) (citrus greening disease); this is considered to be the most serious bacterial disease of citrus plants.

Here we detail an anatomical study of the external and internal anatomy (excluding the reproductive system) using micro-computed tomography (micro-CT). This is the first complete 3D micro-CT reconstruction o... Read more

Javier Alba-Tercedor, Wayne B. Hunter & Ignacio Alba-Alejandre

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3D visualization and deep-learning reveal complex parasite networks in behaviorally manipulated ants

3D visualization and deep-learning reveal complex parasite networks in behaviorally manipulated ants

Microbial parasites may behave collectively to manipulate their host’s behavior. We examine adaptations of a microbial parasite in its natural environment: the body of its coevolved and manipulated host.

Electron microscopy and 3D reconstructions of host and parasite tissues reveal that this fungus invades muscle fibers throughout the ant’s body but leaves the brain intact, and that the fungal cells connect to form extensive networks.

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Maridel A. Fredericksena, Yizhe Zhangb, Missy L. Hazenc, Raquel G. Loretoa,d, Colleen A. Mangoldd,e, Danny Z. Chenb, and David P. Hughes, Department of Entomology, Pennsylvania State University

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A correlative approach for combining microCT, light and transmission electron microscopy in a single 3D scenario

A correlative approach for combining microCT, light and transmission electron microscopy in a single 3D scenario

In biomedical research, a huge variety of different techniques is currently available for the structural examination of small specimens, including conventional light microscopy (LM), transmission electron microscopy (TEM), confocal laser scanning microscopy (CLSM), microscopic X-ray computed tomography (microCT), and many others. Since every imaging method is physically limited by certain parameters, a correlative use of complementary methods often yields a significant broader range of inform... Read more

Stephan Handschuh, Natalie Baeumler, Thomas Schwaha and Bernhard Ruthensteiner

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Paleozoic Nymphal Wing Pads Support Dual Model of Insect Wing Origins

Paleozoic Nymphal Wing Pads Support Dual Model of Insect Wing Origins

The appearance of wings in insects, early in their evolution [1], has been one of the more critical innovations contributing to their extraordinary diversity. Despite the conspicuousness and importance of wings, the origin of these structures has been difficult to resolve and represented one of the “abominable mysteries” in evolutionary biology [2]. More than a century of debate has boiled the matter down to two competing alternatives—one of wings representing an extension of the thorac... Read more

Department of Zoology, Faculty of Science, Charles University, Praha, Czech Republic and al.

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3D Muscle Architecture of the Pectoral Muscles of European Starling (Sturnus vulgaris)

3D Muscle Architecture of the Pectoral Muscles of European Starling (Sturnus vulgaris)

Avian flight is achieved through a number of modifications to the body, including the pectoral girdle (…). Muscle architecture is a critical variable in determining the biomechanical function of the vertebrate musculoskeletal system; however, accurate three-dimensional (3D) understanding of muscle architecture has been historically difficult to acquire. Here, we present a musculoskeletal model of a European starling (Sturnus vulgaris) pectoral girdle generated from iodine contr... Read more

S.P. Sullivan, F.R. McGechie, K.M. Middleton, C.M. Holliday

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Testing hypotheses for the function of the carnivoran baculum using finite-element analysis

Testing hypotheses for the function of the carnivoran baculum using finite-element analysis

The baculum (os penis) is a mineralized bone within the glans of the mammalian penis and is one of the most morphologically diverse structures in the mammal skeleton. (…) For the first time, to our knowledge, we apply a computational simulation approach (finite-element analysis; FEA) to quantify the three-dimensional biomechanical performance of carnivoran bacula (n = 74) based upon high-resolution micro-computed tomography scans. (…) a highly significant negative relation... Read more

Charlotte A. Brassey, James D. Gardiner, and Andrew C. Kitchener

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Caudals and Calyces: The Curious Case of a Consumed Chiapan Colubroid

Caudals and Calyces: The Curious Case of a Consumed Chiapan Colubroid

A new genus and species of colubroid snake is described from the isolated highlands of western Chiapas. This enigmatic little snake possesses a unique suite of characters that defies placing it in any known genus and clearly distinguishes it from all known genera. Several of the most unusual features include subcaudals undivided throughout the length of the tail and a simple hemipenis completely adorned with calyces and having a sulcus spermaticus that remains unbifurcated until the apical po... Read more

Jonathan A. Campbell, Eric N. Smith, and Alexander S. Hall

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Comparative Morphology of the Internal Nasal Skeleton of Adult Marsupials Based on X-ray Computed Tomography

Comparative Morphology of the Internal Nasal Skeleton of Adult Marsupials Based on X-ray Computed Tomography

The internal skeleton of the nasal cavity is sporadically and often incompletely described for many marsupial species and mammals in general. Here, I provide an anatomical survey of the ethmoid in the skulls of adult marsupials based on examination of computed tomography (CT) imagery of 29 taxa representing all the major extant groups of marsupials. (…) I compared ecological data with the number of ecto- and endoturbinals as a preliminary test to determine whether some of the interspec... Read more

Thomas E. Macrini

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Observing the devastating coffee berry borer (Hypothenemus hampei) inside the coffee berry using microcomputed tomography

Observing the devastating coffee berry borer (Hypothenemus hampei) inside the coffee berry using microcomputed tomography

The coffee berry borer is the most devastating insect pest of coffee throughout the world. The insect spends most of its life cycle inside the coffee berry, which makes it quite difficult to observe its behaviour. Micro-computed tomography (micro-CT) was used to observe all developmental stages of the coffee berry borer inside coffee berries (Coffea canephora). An interesting oviposition pattern involving a sequential placement of eggs starting in the periphery of the seed and moving inwards ... Read more

Ignacio Alba-Alejandre, Javier Alba-Tercedor, Fernando E. Vega

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Oldest skeleton of a fossil flying squirrel casts new light on the phylogeny of the group

Oldest skeleton of a fossil flying squirrel casts new light on the phylogeny of the group

Here we report the oldest fossil skeleton of a flying squirrel (11.6 Ma) that displays the gliding-related diagnostic features shared by extant forms and allows for a recalibration of the divergence time between tree and flying squirrels. Our phylogenetic analyses combining morphological and molecular data generally support older dates than previous molecular estimates (~23 Ma), being congruent with the inclusion of some of the earliest fossils (~36 Ma) into this clade. They also show that fl... Read more

Isaac Casanovas-Vilar, Joan Garcia-Porta, Josep Fortuny, Oscar Sanisidro, Jerome Prieto, Marina Querejeta, Sergio Llacer, Josep M Robles, Federico Bernardini, David M Alba

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Vascular structure of the earliest shark teeth

Vascular structure of the earliest shark teeth

Here we use synchrotron tomography to characterise dental vasculature in the oldest known tooth-bearing sharks, Leonodus carlsi Mader, 1986 and Celtiberina maderi Wang, 1993. Three dimensional reconstruction of the vascular system and microstructure of both taxa revealed a complex and dense network of canals, including horizontal, ascending and secondary bifurcated canals, as well as histological features consistent with an osteodont histotype. However, L. carlsi and C. maderi also exhibit si... Read more

Carlos Martinez-Perez, Alba Martin-Lazaro, Humberto G Ferron, Martina Kirstein, Philip C.J. Donoghue, Hector Botella

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Digitizing extant bat diversity: An open-access repository of 3D μCT-scanned skulls for research and education

Digitizing extant bat diversity: An open-access repository of 3D μCT-scanned skulls for research and education

Biological specimens are primary records of organismal ecology and history. As such, museum collections are invaluable repositories for testing ecological and evolutionary hypotheses across the tree of life. Digitizing and broadly sharing the phenotypic data from these collections serves to expand the traditional reach of museums, enabling widespread data sharing, collaboration, and education at an unprecedented scale. In recent years, μCT-scanning has been adopted as one way for efficiently... Read more

Jeff J. Shi, Erin P. Westeen, Daniel L. Rabosky

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Morphology of the bryozoan Cinctipora elegans (Cyclostomata, Cinctiporidae) with first data on its sexual reproduction and the cyclostome neuro-muscular system

Morphology of the bryozoan Cinctipora elegans (Cyclostomata, Cinctiporidae) with first data on its sexual reproduction and the cyclostome neuro-muscular system

Cyclostome bryozoans are an ancient group of marine colonial suspension-feeders comprising approximately 700 extant species. Previous morphological studies are mainly restricted to skeletal characters whereas data on soft tissues obtained by state-of-the-art methods are still lacking. In order to contribute to issues related to cyclostome ground pattern reconstruction, we analyzed the morphology of the neuromuscular system Cinctipora elegans by means of immunocytochemical staining,... Read more

Thomas F. Schwaha, Stephan Handschuh, Andrew N. Ostrovsky, Andreas Wanninger

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The morphological diversity of Garra barreimiae

The morphological diversity of Garra barreimiae

Garra barreimiae (Fowler and Steinitz, Bull Res Counc Isr 5B:262–289, 1956) is a freshwater fish that lives throughout the Hajar Mountains of Oman and the United Arab Emirates. Previously, four different genetic clades (West clade, C... Read more

Arthur Pichler; Harald Ahnelt; Sandra Kirchner; Helmut Sattmann; Elisabeth Haring; Stephan Handschuh; Jörg Freyhof; Reginald Victor; Luise Kruckenhauser

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Morphology and ontogeny of Lophopus crystallinus lophophore support the epistome as ancestral character of phylactolaemate bryozoans

Morphology and ontogeny of Lophopus crystallinus lophophore support the epistome as ancestral character of phylactolaemate bryozoans

Phylactolaemate bryozoans are the sister-group to all remaining bryozoan taxa. Consequently, their study is essential to reveal and analyze ancestral traits of Phylactolaemata and Bryozoa in general.

They are the only bryozoans to possess an epistome which traditionally has been regarded as shared with phoronids and brachiopods. Contrary to older observations, an epistome was recently reported to be missing in the early branching phylactolaemate Lophopus crystallinus. In this... Read more

Thomas Schwaha

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Pneumatisation and internal architecture of the Southern Cassowary Casuarius casuarius casque: a microCT study

Pneumatisation and internal architecture of the Southern Cassowary Casuarius casuarius casque: a microCT study

Cranial ornaments such as keratinous horns and bony casques are commonplace amongst birds and take a variety of diverse forms. Possible functions include display, thermoregulation, vocalisation and intraspecific combat, yet few hypotheses have been directly tested. Here we investigate the anatomy and mechanics of the casque of the Southern Cassowary (Casuarius casuarius), and test functional hypotheses using CT-based virtual dissection.

In particular, we determine the nature of pneumat... Read more

Charlotte A. Brassey , Thomas O’Mahoney

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Evolutionary radiation of earless frogs in the Andes: molecular phylogenetics and habitat shifts in high-elevation terrestrial breeding frogs

Evolutionary radiation of earless frogs in the Andes: molecular phylogenetics and habitat shifts in high-elevation terrestrial breeding frogs

The loss of hearing structures and loss of advertisement calls in many terrestrial breeding frogs (Strabomantidae) living at high elevations in South America are common and intriguing phenomena. The Andean frog genus Phrynopus Peters, 1873 has undergone an evolutionary radiation in which most species lack the tympanic membrane and tympanic annulus, yet the phylogenetic relationships among species in this group remain largely unknown. Here, we present an expanded molecular phylogeny o... Read more

May R, Lehr E, Rabosky DL. PeerJ 6:e4313

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The NOVA project: maximizing beam time efficiency through synergistic analyses of SRµCT data

The NOVA project: maximizing beam time efficiency through synergistic analyses of SRµCT data

Beamtime and resulting SRμCT data are a valuable resource for researchers of a broad scientific community in life sciences. Most research groups, however, are only interested in a specific organ and use only a fraction of their data. The rest of the data usually remains untapped. By using a new collaborative approach, the NOVA project (Network for Online Visualization and synergistic Analysis of tomographic data) aims to demonstrate, that more efficient use of the valuable beam time is possi... Read more

The international society for optics and photonics (SPIE)

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