Welcome to the Amira-Avizo Software Use Case Gallery

Below you will find a collection of use cases of our 3D data visualization and analysis software. These use cases include scientific publications, articles, papers, posters, presentations or even videos that show how Amira-Avizo Software is used to address various scientific and industrial research topics.

Use the Domain selector to filter by main application area, and use the Search box to enter keywords related to specific topics you are interested in.

Computational fluid dynamics with imaging of cleared tissue and of in vivo perfusion predicts drug uptake and treatment responses in tumours

Computational fluid dynamics with imaging of cleared tissue and of in vivo perfusion predicts drug uptake and treatment responses in tumours

Understanding the uptake of a drug by diseased tissue, and the drug’s subsequent spatiotemporal distribution, are central factors in the development of effective targeted therapies. However, the interaction between the pathophysiology of diseased tissue and individual therapeutic agents can be complex, and can vary across tissue types and across subjects. Here, we show that the combination of mathematical modelling, high-resolution optical imaging of intact and optically cleared tumour tiss... Read more

Angela d’Esposito, Paul W. Sweeney, Morium Ali, Magdy Saleh, Rajiv Ramasawmy, Thomas A. Roberts, Giulia Agliardi, Adrien Desjardins, Mark F. Lythgoe, R. Barbara Pedley, Rebecca Shipley and Simon Walker-Samuel

Read paper
Step-by-step guide to post-acquisition correlation of confocal and FIB/SEM volumes using Amira software

Step-by-step guide to post-acquisition correlation of confocal and FIB/SEM volumes using Amira software

In recent years new methodologies and workflow pipelines for acquiring correlated fluorescence microscopy and volume electron microscopy datasets have been extensively described and made accessible to users of different levels. Post-acquisition image processing, and particularly correlation of the optical and electron data in a single integrated three-dimensional framework can be key for extracting valuable information, especially when imaging large sample volumes such as whole cells or tissu... Read more

Allon Weiner

Learn more
A multianalytic investigation of weapon-related injuries in a Late Antiquity necropolis, Mutina, Italy

A multianalytic investigation of weapon-related injuries in a Late Antiquity necropolis, Mutina, Italy

Human skeletal remains from archaeological contexts occasionally present signs of traumatic injuries from weapons, revealing, for example, the degree of interpersonal violence, the type of weapon and the sequence of events of a specific historical context.

Traumatic lesions are generally analyzed using macroscopic and microscopic methods, which are not necessarily integrated in the same study. In this study, we employed a multi-analytical approach to determine i... Read more

Antonino Vazzana, Lucia Martina Scalise, Mirko Traversari, Carla Figus, Salvatore Andrea Apicella, Laura Buti, Gregorio Oxilia, Rita Sorrentino, Silvia Pellegrini, Chiara Matteucci, Lucio Calcagnile, Raffaele Savigni, Robin N.M.Feeney, Giorgio Gruppioni, Stefano Benazziah

Read paper
VascuViz: a multimodality and multiscale imaging and visualization pipeline for vascular systems biology

VascuViz: a multimodality and multiscale imaging and visualization pipeline for vascular systems biology

Despite advances in imaging, image-based vascular systems biology has remained challenging because blood vessel data are often available only from a single modality or at a given spatial scale, and cross-modality data are difficult to integrate.

Therefore, there is an exigent need for a multimodality pipeline that enables ex vivo vascular imaging with magnetic resonance imaging, computed tomography and optical microscopy of the same sample, while permitting imaging with complementary c... Read more

Akanksha Bhargava, Benjamin Monteagudo, Priyanka Kushwaha, Janaka Senarathna, Yunke Ren, Ryan C. Riddle , Manisha Aggarwal and Arvind P. Pathak

Read paper
Morphological evolution and internal strain mapping of pomelo peel using X-ray computed tomography and digital volume correlation

Morphological evolution and internal strain mapping of pomelo peel using X-ray computed tomography and digital volume correlation

Among natural cellular materials, pomelo peels, having a foam-like hierarchical microstructure, represent an ideal model for developing materials with high energy absorption efficiency. In this work, by combining X-ray tomographic imaging technique and digital volume correlation (DVC), in-situ stepwise uniaxial compression tests were performed to quantify the internal morphological evolution and kinematic responses of pomelo peel samples during compression.

Read more

B.Wang, B.Pan, G.Lubineau

Read paper
Three-dimensional grain mapping of open-cell metallic foam by integrating synthetic data with experimental data from high-energy X-ray diffraction microscopy

Three-dimensional grain mapping of open-cell metallic foam by integrating synthetic data with experimental data from high-energy X-ray diffraction microscopy

The complex mechanical response of open-cell foams depends strongly on the hierarchy of length scales inherent in them, from engineering-part scale to the ligament scale through the grain scale down to the crystal-lattice scale. A first step toward understanding and predicting the coordinated mechanical response across le... Read more

Jayden C. Plumb, Jonathan F. Lind, Joseph C. Tucker, Ron Kelley, Ashley D. Spear

Read paper
Over the rainbow? Micro-CT scanning to non-destructively study Roman and early medieval glass bead manufacture

Over the rainbow? Micro-CT scanning to non-destructively study Roman and early medieval glass bead manufacture

The usefulness of desktop Micro-CT scanners for the study of archaeological artefacts is demonstrated in a non-destructive study of manufacturing methods of Roman and Early Medieval monochrome and polychrome glass beads. Differences in glass colours show up in these scans as differences in attenuation. The presence and distribution of bubbles and various inclusions (metal, opacifier) are also well visible. Shaft shapes and patterns of bubbles inside the glass make it possible in most cases to... Read more

D.J.M.Ngan-Tillard, D.J.Huisman, F.Corbella, A.Van Nass

Read paper
Improving microstructural quantification in FIB/SEM nanotomography

Improving microstructural quantification in FIB/SEM nanotomography

Advanced nanotomographic analysis is still far from routine, and a number of challenges remain in data acquisition and post-processing. In this work, we present a number of techniques to improve the quality of the acquired data, together with easy-to-implement methods to obtain “advanced” microstructural quantifications. The techniques are applied to a solid oxide fuel cell cathode of interest to the electrochemistry community, but the methodologies are easily adaptable to a wide range of... Read more

Joshua A.Taillon, Christopher Pellegrinelli, Yi-Lin Huang, Eric D.Wachsman, Lourdes G. Salamanca-Riba

Read paper
Investigation of Hot Pressed Polymer Electrolyte Fuel Cell Assemblies via X-ray Computed Tomography

Investigation of Hot Pressed Polymer Electrolyte Fuel Cell Assemblies via X-ray Computed Tomography

The hot pressing process for fabricating membrane electrode assemblies (MEAs) has been widely adopted, yet little is known of its effects on the microstructural properties of the different components of the MEA. In particular, the interaction of the electrolyte, electrode and gas diffusion layer (GDL) due to lamination is difficult to probe as conventional imaging techniques cannot access the internal structure of the MEA. Here, a novel approach is used, which combines characterisation of hot... Read more

Q. Meyer, N. Mansor, F. Iacoviello, P.L. Cullen, R. Jervis, D. Finegan, C. Tan, J. Bailey, P. R. Shearing, D. J. L. Brett

Read paper
Characterization of the bone marrow adipocyte niche with three-dimensional electron microscopy

Characterization of the bone marrow adipocyte niche with three-dimensional electron microscopy

Unlike white and brown adipose tissues, the bone marrow adipocyte (BMA) exists in a microenvironment containing unique populations of hematopoietic and skeletal cells.

To study this microenvironment at the subcellular level, we performed a three-dimensional analysis of the ultrastructure of the BMA niche with focused ion beam scanning electron microscopy (FIB-SEM). This revealed that BMAs display hallmarks of metabolically active cells including polarized lipid deposits, a dense mitoch... Read more

Hero Robles, SungJae Park, Matthew S. Joens, James A.J. Fitzpatrick, Clarissa S. Craft, Erica L. Scheller

Read paper
In Situ Synthesized La0.6Sr0.4Co0.2Fe0.8O3−δ–Gd0.1Ce0.9O1.95 Nanocomposite Cathodes via a Modified Sol–Gel Process for Intermediate Temperature Solid Oxide Fuel Cells

In Situ Synthesized La0.6Sr0.4Co0.2Fe0.8O3−δ–Gd0.1Ce0.9O1.95 Nanocomposite Cathodes via a Modified Sol–Gel Process for Intermediate Temperature Solid Oxide Fuel Cells

Composite cathodes comprising nanoscale
powders are expected to impart with high specific surface
area and triple phase boundary (TPB) density, which will lead
to better performance.

However, uniformly mixing nanosized heterophase powders remains a challenge due to their high surface energy and thus ease with which they agglomerate into their individual phases during the mixing and sintering
processes. In this study, we successfully synthesized La0.6Sr0.4Co0.2Fe... Read more

Dong Woo Joh, Areum Cha, Jeong Hwa Park, Kyeong Joon Kim, Kyung Taek Bae, Doyeub Kim, Young Ki Choi, Hyegsoon An, Ji Su Shin, Kyung Joong Yoon, and Kang Taek Lee

Read paper
High-speed synchrotron X-ray imaging of glass foaming and thermal conductivity simulation

High-speed synchrotron X-ray imaging of glass foaming and thermal conductivity simulation

Glass foams are attractive thermal insulation materials, thus, the thermal conductivity (λ) is crucial for their insulating performance. Understanding the foaming process is critical for process optimization. Here, we applied high-speed synchrotron X-ray tomography to investigate the change in pore structure during the foaming process, quantifying the foam structures and porosity dynamically. The results can provide guidance for the manufacturing of glass foams. The 3D pore structures were a... Read more

Martin B. Østergaard, Manlin Zhang, Xiaomei Shen, Rasmus R. Petersen, Jakob König, Peter D. Lee, Yuanzheng Yue, Biao Cai

Read paper
Impact of Pore Tortuosity on Electrode Kinetics in Lithium Battery Electrodes: Study in Directionally Freeze-Cast LiNi0.8Co0.15Al0.05O2 (NCA)

Impact of Pore Tortuosity on Electrode Kinetics in Lithium Battery Electrodes: Study in Directionally Freeze-Cast LiNi0.8Co0.15Al0.05O2 (NCA)

The prevailing electrode fabrication method for lithium-ion battery electrodes includes calendering at high pressures to densify the electrode and promote adhesion to the metal current collector.

However, this process increases the tortuosity of the pore network in the primary transport direction and imposes severe tradeoffs between electrode thickness and rate capability. With the aim of understanding the impact of pore tortuosity on electrode kinetics, and enabling cell designs with ... Read more

Benjamin Delattre, Ruhul Amin, Jonathan Sander, Joël De Coninck, Antoni P. Tomsia and Yet-Ming Chiang

Read full paper
Regional diversity in the murine cortical vascular network is revealed by synchrotron X-ray tomography and is amplified with age

Regional diversity in the murine cortical vascular network is revealed by synchrotron X-ray tomography and is amplified with age

Cortical bone is permeated by a system of pores, occupied by the blood supply and osteocytes. With ageing, bone mass reduction and disruption of the microstructure are associated with reduced vascular supply. Insight into the regulation of the blood supply to the bone could enhance the understanding of bone strength determinants and fracture healing. Using synchrotron radiation-based computed tomography, the distribution of vascular canals and osteocyte lacunae was assessed in murine cortica... Read more

J.A. Núñez; A. Goring; B. Javaheri; H. Razi; D. Gomez-Nicola; E. Hesse; A.A. Pitsillides; P.J. Thurner; P. Schneider; E. Clarkin

Read full paper
Three-dimensional thermal stress analysis of the re-oxidized Ni-YSZ anode functional layer in solid oxide fuel cells

Three-dimensional thermal stress analysis of the re-oxidized Ni-YSZ anode functional layer in solid oxide fuel cells

Nickel-yttria-stabilized zirconia (Ni-YSZ) cermet is widely used as an anode material in solid oxide fuel cells (SOFCs); however, Ni re-oxidation causes critical problems due to volume expansion, which causes high thermal stress. We fabricated a Ni-YSZ anode functional layer (AFL), which is an essential component in high-performance SOFCs, and re-oxidized it to investigate the related three-dimensional (3D) microstructural and thermo-mechanical effects. A 3D model of the re-oxidized AFL ... Read more

Jun Woo Kim, Kiho Bae, Hyun Joong Kim, Ji-won Son, Namkeun Kim, Stefan Stenfelt, Fritz B. Prinz, Joon Hyung Shim

Read paper
A three-dimensional heterogeneity analysis of electrochemical energy conversion in SOFC anodes using electron nanotomography and mathematical modeling

A three-dimensional heterogeneity analysis of electrochemical energy conversion in SOFC anodes using electron nanotomography and mathematical modeling

In this paper a fully three dimensional, multiphase, micro-scale solid oxide fuel cellanode transport phenomena numerical model is proposed and verified. The Butler-Volmer model was combined with empirical relations for conductivity and diffusivity – notably the Fuller-Shetler-Giddings equation, and the Fickian modelfor transport of gas reagents. FIB-SEM tomography of a commercial SOFC stack anode was performed and the resulting images were processed to acquire input data. ... Read more

Tomasz A. Prokop, Katarzyna Berent, Hiroshi Iwai, Janusz S.Szmyd, Grzegorz Brus

Read paper
X-ray computed tomography comparison of individual and parallel assembled commercial lithium iron phosphate batteries at end of life after high rate cycling

X-ray computed tomography comparison of individual and parallel assembled commercial lithium iron phosphate batteries at end of life after high rate cycling

X-ray computed tomography (X-ray CT) across multiple length scales is utilized for the first time to investigate the physical abuse of high C-rate pulsed discharge on cells wired individually and in parallel.. Manufactured lithium iron phosphate cells boasting high rate capability were pulse power tested in both wiring conditions with high discharge currents of 10C for a high number of cycles (up to 1200) until end of life (<80% of initial discharge capacity retained). The parallel ass... Read more

Rachel Carter, Brett Huhman, Corey T. Love, Iryna V. Zenyuk

Read paper
Enhanced Water Management of Polymer Electrolyte Fuel Cells with Additive-Containing Microporous Layers.

Enhanced Water Management of Polymer Electrolyte Fuel Cells with Additive-Containing Microporous Layers.

This work describes the performance improvement of a polymer electrolyte fuel cell with a novel class of microporous layers (MPLs) that incorporates hydrophilic additives: one with 30 μm aluminosilicate fibers and another with multiwalled carbon nanotubes with a domain size of 5 μm. Higher current densities at low potentials were observed for cells with the additive-containing MPLs compared to a baseline cell with a conventional MPL, which correlate with improvements in water management. Th... Read more

Dusan Spernjak, Rangachary Mukundan, Rodney L. Borup, Liam Connolly, Benjamin Zackin, Vincent De Andrade, Michael Wojcik, Dilworth Y. Parkinson, David Jacobson, Daniel Seth Hussey, Karren L More, Thomas Chan, Adam Z Weber, and Iryna V. Zenyuk

Read paper
High performance anode with dendritic porous structure for low temperature solid oxide fuel cells

High performance anode with dendritic porous structure for low temperature solid oxide fuel cells

A dendritic porous supported microstructure simultaneously creates small pore size and broad gas diffusion pathways in a solid oxide fuel cell anode membrane. This microstructure also achieves pore sizes that reduce with increasing depth within the membrane without increasing the structure tortuosity. Such a microstructure supplies high triple phase boundary density, fast gas diffusion and low polarization resistance. Here we characterise the performance of a porous anode with such a dendriti... Read more

Xin Shao, William D.A. Rickard, Dehua Dong, Huu Dang , Martin Saunders, Aaron Dodd, Gordon Parkinson, Chun-Zhu Li

Read paper
A numerical analysis of unsteady transport phenomena in a Direct Internal Reforming Solid Oxide Fuel Cell

A numerical analysis of unsteady transport phenomena in a Direct Internal Reforming Solid Oxide Fuel Cell

Growing popularity and rapid development of Solid Oxide Fuel Cells (SOFCs) stem for their potential to become a gamechanger in the field of clean power generation technologies.

In this paper, a transient microstructure-oriented numerical simulation of a planar Direct Internal Reforming Solid Oxide Fuel Cell (DIR-SOFC) is delivered. The performance criteria in a direct steam reforming for a fuel starvation scenario are analyzed in order to optimize the underlying process. The proposed t... Read more

Maciej Chalusiak, Michal Wrobel, Marcin Mozdzierz, Katarzyna Berent, Janusz S. Szmyd, Shinji Kimijima, Grzegorz Brus

Read paper
4D imaging of polymer electrolyte membrane fuel cell catalyst layers by soft X-ray spectro-tomography

4D imaging of polymer electrolyte membrane fuel cell catalyst layers by soft X-ray spectro-tomography

4D imaging – the three-dimensional distributions of chemical species determined using multi-energy X-ray tomography – of cathode catalyst layers of polymer electrolyte membrane fuel cells (PEM-FC) has been measured by scanning transmission x-ray microscopy (STXM) spectro-tomography at the C 1s and F 1s edges. In order to monitor the effects of radiation damage on the composition and 3D structure of the perfluorosulfonic acid (PFSA) ionomer, the same volume was measu... Read more

Juan Wu, Lis G.A.Melo, Xiaohui Zhu, Marcia M.West, Viatcheslav Berejnov, Darija Susac, Juergen Stumper, Adam P.Hitchcock

Read paper