Welcome to the Amira-Avizo Software Use Case Gallery

Below you will find a collection of use cases of our 3D data visualization and analysis software. These use cases include scientific publications, articles, papers, posters, presentations or even videos that show how Amira-Avizo Software is used to address various scientific and industrial research topics.

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STIM1 promotes migration, phagosomal maturation and antigen cross-presentation in dendritic cells

STIM1 promotes migration, phagosomal maturation and antigen cross-presentation in dendritic cells

Antigen cross-presentation by dendritic cells (DC) stimulates cytotoxic T cell activation to promote immunity to intracellular pathogens, viruses and cancer. Phagocytosed antigens generate potent T cell responses, but the signalling and trafficking pathways regulating their cross-presentation are unclear. Here, we show that ablation of the store-operated-Ca2+-entry regulator STIM1 in mouse myeloid cells impairs cross-presentation and DC migration in vivo and in vitro. Stim1... Read more

Paula Nunes-Hasler, Sophia Maschalidi, Carla Lippens, Cyril Castelbou, Samuel Bouvet, Daniele Guido, Flavien Bermont, Esen Y. Bassoy, Nicolas Page, Doron Merkler, Stéphanie Hugues, Denis Martinvalet, Bénédicte Manoury & Nicolas Demaurex

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Morphology of the bryozoan Cinctipora elegans (Cyclostomata, Cinctiporidae) with first data on its sexual reproduction and the cyclostome neuro-muscular system

Morphology of the bryozoan Cinctipora elegans (Cyclostomata, Cinctiporidae) with first data on its sexual reproduction and the cyclostome neuro-muscular system

Cyclostome bryozoans are an ancient group of marine colonial suspension-feeders comprising approximately 700 extant species. Previous morphological studies are mainly restricted to skeletal characters whereas data on soft tissues obtained by state-of-the-art methods are still lacking. In order to contribute to issues related to cyclostome ground pattern reconstruction, we analyzed the morphology of the neuromuscular system Cinctipora elegans by means of immunocytochemical staining,... Read more

Thomas F. Schwaha, Stephan Handschuh, Andrew N. Ostrovsky, Andreas Wanninger

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Three-dimensional virtual histology of human cerebellum by X-ray phase-contrast tomography

Three-dimensional virtual histology of human cerebellum by X-ray phase-contrast tomography

To quantitatively evaluate brain tissue and its corresponding function, knowledge of the 3D cellular distribution is essential. The gold standard to obtain this information is histology, a destructive and labor-intensive technique where the specimen is sliced and examined under a light microscope, providing 3D information at nonisotropic resolution. To overcome the limitations of conventional histology, we use phase-contrast X-ray tomography with optimized optics, reconstruction, and image an... Read more

Mareike Töpperwien, Franziska van der Meer, Christine Stadelmann, and Tim Salditt

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3D Reconstruction of Lipid Droplets in the Seed of Brassica napus

3D Reconstruction of Lipid Droplets in the Seed of Brassica napus

Rapeseed is one of the most important and widely cultured oilseed crops for food and nonfood purposes worldwide. Neutral lipids are stored in lipid droplets (LDs) as fuel for germination and subsequent seedling growth. Most of the LD detection in seeds was still in 2D levels, and some of the details might have been lost in previous studies. In the present work, the configuration of LDs in seeds was obtained by confocal imaging combined with 3D reconstruction technology in Brassica napus<... Read more

Yongtai Yin, Liangxing Guo, Kang Chen, Zhenyi Guo, Hongbo Chao, Baoshan Wang, and Maoteng Licorresponding author

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Stochastic reconstruction of carbon fiber paper gas diffusion layers of PEFCs: A comparative study

Stochastic reconstruction of carbon fiber paper gas diffusion layers of PEFCs: A comparative study

Fuel cells are electrochemical devices that convert the chemical energy of fuels into electrical energy. An ideal option for a wide variety of portable, stationary, and automotive applications is using the polymer electrolyte
fuel cells due to their modular design, high efficiency, and environmental benefits.

A 3D microstructure of the non-woven gas diffusion layers (GDLs) of polymer electrolyte fuel cells (PEFCs) is reconstructed using a stochastic method. For a commercial GDL, ... Read more

Sepehr Sima Afrookhteh, Jalil Jamali, Mohsen Shakeric, Majid Baniassadi

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Drying of Corn Kernels: From Experimental Images to Multiscale Multiphysics Modeling

Drying of Corn Kernels: From Experimental Images to Multiscale Multiphysics Modeling

This work demonstrated the importance and feasibility of experimental image
to simulation workflow. The workflow is successfully applied to a food processing study, where multiphysics and multiscale modeling
based on 3D experimental image reconstruction contributes to the preservation of corn, one of the major food sources for the world population.

Corn kernels have a complex structure as they are composed of a pericarp layer outside and contain hard and soft endosperm and ... Read more

Pawan S. Takhar, and Shuang Zhang

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Morphology and ontogeny of Lophopus crystallinus lophophore support the epistome as ancestral character of phylactolaemate bryozoans

Morphology and ontogeny of Lophopus crystallinus lophophore support the epistome as ancestral character of phylactolaemate bryozoans

Phylactolaemate bryozoans are the sister-group to all remaining bryozoan taxa. Consequently, their study is essential to reveal and analyze ancestral traits of Phylactolaemata and Bryozoa in general.

They are the only bryozoans to possess an epistome which traditionally has been regarded as shared with phoronids and brachiopods. Contrary to older observations, an epistome was recently reported to be missing in the early branching phylactolaemate Lophopus crystallinus. In this... Read more

Thomas Schwaha

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Flexible all-fiber electrospun supercapacitor

Flexible all-fiber electrospun supercapacitor

Electrospun all-fiber flexible supercapacitor with nanofiber electrodes/separator.

  • Increased graphitic degree with the addition of MnACAC and thermal decomposition.
  • Enhanced capacitive performance with the addition of MnO.
  • Quantified nanofiber alignment and increased bias with MnO over undoped fibers.
  • FIBSEM tomography of nanofibers showing MnO disitribution in carbon nanofibers.

We present an all-fiber flexible supercapacitor with compo... Read more

Xinhua Liu, Max Naylor Marlow, Samuel J. Cooper, Bowen Song, Xiaolong Chen, Nigel P. Brandon, Billy Wu

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Reinvestigating an enigmatic Late Cretaceous monocot: morphology, taxonomy, and biogeography of Viracarpon

Reinvestigating an enigmatic Late Cretaceous monocot: morphology, taxonomy, and biogeography of Viracarpon

Angiosperm-dominated floras of the Late Cretaceous are essential for understanding the evolutionary, ecological, and geographic radiation of flowering plants.

The Late Cretaceous–early Paleogene Deccan Intertrappean Beds of India contain angiosperm-dominated plant fossil assemblages known from multiple localities in central India. Numerous monocots have been documented from these assemblages, providing a window into an important but poorly understood time in their diversification. On... Read more

Kelly K.S. Matsunaga, Selena Y. Smith, Steven R. Manchester, Dashrath Kapgate, Deepak Ramteke, Amin Garbout, and Herminso Villarraga-Gómez

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Cellular in vivo 3D imaging of the cornea by confocal laser scanning microscopy

Cellular in vivo 3D imaging of the cornea by confocal laser scanning microscopy

We present an in vivo confocal laser scanning microscopy based method for large 3D reconstruction of the cornea on a cellular level with cropped volume sizes up to 266 x 286x 396 µm3.

The microscope objective used is equipped with a piezo actuator for automated, fast and precise closed-loop focal plane control. Furthermore, we present a novel concave surface contact cap, which significantly reduces eye movements by up to 87%, hence increasing the overlapping image area of the whole st... Read more

Sebastian Bohn, Karsten Sperlich, Stephan Allgeier, Andreas Bartschat, Ruby Prakasam, Klaus-martin Reichert, Heinrich Stolz, Rudolf Guthoff, Ralf Mikut, Bernd Köhler, and Oliver Stachs

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A Middle Triassic pachypleurosaur (Diapsida: Eosauropterygia) from a restricted carbonate ramp in the Western Carpathia

A Middle Triassic pachypleurosaur (Diapsida: Eosauropterygia) from a restricted carbonate ramp in the Western Carpathia

An eosauropterygian skeleton found in the Middle Triassic (upper Anisian) Gutenstein Formation of the Fatric Unit (Demänovská dolina Valley, Low Tatra Mountains, Slovakia) represents the earliest known occurrence of marine tetrapods in the Western Carpathians. The specimen represents a partly articulated portion of the postcranial skeleton (nine dorsal vertebrae, coracoid, ribs, gastral ribs, pelvic girdle, femur and one zeugopodial element). It is assigned to the Pachypleurosauria, more pr... Read more

ANDREJ ČERŇANSKÝ, NICOLE KLEIN, JÁN SOTÁK, MÁRIO OLŠAVSKÝ, JURAJ ŠURKA, and PAVEL HERICH

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Revealing the skeleton of polar dinosaur using synchrotron computed tomography

Revealing the skeleton of polar dinosaur using synchrotron computed tomography

Leaellynasaura amicagraphica was a small, bipedal, herbivorous dinosaur from the Early Cretaceous (~106 million years ago) of Australia within the Antarctic Circle of that time.

Synchrotron scans, and the resulting 3D reconstruction of the skull and post-cranial skeleton, provide a unique view of the morphology of Leaellynasaura, and allows this material to be 3D printed for display.

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1University College London, UK; Monash University, Melbourne, Australia; Museum Victoria, Melbourne, Australia

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Potential hominin affinities of Graecopithecus from the Late Miocene of Europe

Potential hominin affinities of Graecopithecus from the Late Miocene of Europe

The split of our own clade from the Panini is undocumented in the fossil record. To fill this gap we investigated the dentognathic morphology of Graecopithecus freybergi from Pyrgos Vassilissis (Greece) and cf. Graecopithecus sp. from Azmaka (Bulgaria), using new μCT and 3D reconstructions of the two known specimens. Pyrgos Vassilissis and Azmaka are currently dated to the early Messinian at 7.175 Ma and 7.24 Ma. Mainly based on its external preservation and the previou... Read more

Jochen Fuss, Nikolai Spassov, David R. Begun, Madelaine Böhme

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A new ankylosaurine dinosaur from the Judith River Formation of Montana, USA, based on an exceptional skeleton with soft tissue preservation

A new ankylosaurine dinosaur from the Judith River Formation of Montana, USA, based on an exceptional skeleton with soft tissue preservation

The terrestrial Judith River Formation of northern Montana was deposited over an approximately 4 Myr interval during the Campanian (Late Cretaceous). Despite having been prospected and collected continuously by palaeontologists for over a century, few relatively complete dinosaur skeletons have been recovered from this unit to date. Here we describe a new genus and species of ankylosaurine dinosaur, Zuul crurivastator, from the Coal Ridge Member of the Judith River Formation, based ... Read more

Victoria M. Arbour, David C. Evans

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Analysis of Void Volume in Composite Electrode of All-solid-state Lithium-ion Battery Employing FIB-SEM and Union Operation Image Processing

Analysis of Void Volume in Composite Electrode of All-solid-state Lithium-ion Battery Employing FIB-SEM and Union Operation Image Processing

We had proposed an image-processing scheme using union operation suitable for extracting target features with hierarchical dimensions from the original data, and applied it to void analysis in a composite electrode of an all-solid-state lithium ion battery (LIB). Void analysis is very important in developing better composite electrodes for all-solid-state LIBs because internal voids should increase the interfacial resistance. Film formation of electrode-solid electrolyte composites by the aer... Read more

Yuta Yamamoto , Yasutoshi Iriyama, and Shunsuke Muto

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Severe hearing loss and outer hair cell death in homozygous Foxo3 knockout mice after moderate noise exposure

Severe hearing loss and outer hair cell death in homozygous Foxo3 knockout mice after moderate noise exposure

Noise induced hearing loss (NIHL) is a disease that affects millions of Americans. Identifying genetic pathways that influence recovery from noise exposure is an important step forward in understanding NIHL. The transcription factor Foxo3 integrates the cellular response to oxidative stress and plays a role in extending lifespan in many organisms, including humans. Here we show that Foxo3 is required for auditory function after noise exposure in a mouse model system, measured by ABR…Read more

Felicia Gilels, Stephen T. Paquette, Holly J. Beaulac, Anwen Bullen & Patricia M. White

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Human liver segments: role of cryptic liver lobes and vascular physiology in the development of liver veins and left-right asymmetry

Human liver segments: role of cryptic liver lobes and vascular physiology in the development of liver veins and left-right asymmetry

Couinaud based his well-known subdivision of the liver into (surgical) segments on the branching order of portal veins and the location of hepatic veins. However, both segment boundaries and number remain controversial due to an incomplete understanding of the role of liver lobes and vascular physiology on hepatic venous development. Human embryonic livers (5–10 weeks of development) were visualized with Amira 3D-reconstruction and Cinema 4D-remodeling software.

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Jill P. J. M. Hikspoors, Mathijs M. J. P. Peeters, Nutmethee Kruepunga, Hayelom K. Mekonen, Greet M. C. Mommen, S. Eleonore Köhler & Wouter H. Lamers

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X-ray computed tomography of packed bed chromatography columns for three dimensional imaging and analysis

X-ray computed tomography of packed bed chromatography columns for three dimensional imaging and analysis

Physical characteristics critical to chromatography including geometric porosity and tortuosity within the packed column were analysed based upon three dimensional reconstructions of bed structure in-situ. Image acquisition was performed using two X-ray computed tomography systems, with optimisation of column imaging performed for each sample in order to produce three dimensional representations of packed beds at 3 μm resolution.

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Department of Biochemical Engineering, University College London | Pall Life Sciences, Portsmouth | Electrochemical Innovation Lab, Department of Chemical Engineering, University College London

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Evolutionary radiation of earless frogs in the Andes: molecular phylogenetics and habitat shifts in high-elevation terrestrial breeding frogs

Evolutionary radiation of earless frogs in the Andes: molecular phylogenetics and habitat shifts in high-elevation terrestrial breeding frogs

The loss of hearing structures and loss of advertisement calls in many terrestrial breeding frogs (Strabomantidae) living at high elevations in South America are common and intriguing phenomena. The Andean frog genus Phrynopus Peters, 1873 has undergone an evolutionary radiation in which most species lack the tympanic membrane and tympanic annulus, yet the phylogenetic relationships among species in this group remain largely unknown. Here, we present an expanded molecular phylogeny o... Read more

May R, Lehr E, Rabosky DL. PeerJ 6:e4313

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The Academic Medical Center (AMC) uses Amira to build a 3D Atlas for Human Embryology

The Academic Medical Center (AMC) uses Amira to build a 3D Atlas for Human Embryology

The 3D Atlas of Human Embryology project was funded by the Academic Medical Center (AMC) in Amsterdam, the Netherlands, in 2009. Since then, over 75 students, under the supervision of embryologists of the Department of Anatomy, Embryology & Physiology, have contributed to this labor-int... Read more

Academic Medical Center

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